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http://cicese.repositorioinstitucional.mx/jspui/handle/1007/1690| A new member of the gamma-conotoxin family, isolated from Conus princeps displays a novel molecular target | |
| JOHANNA BERNALDEZ SARABIA | |
| SAMANTA MIREYA JIMENEZ FLORES LUIS JAVIER GONZALEZ ORTIZ Jesús ENRIQUE SOTO EGUIBAR JOSE EMILIO SALCEDA RUANOVA DANIELA SILEM CHAVEZ RAMIREZ MANUEL BENIGNO AGUILAR RAMIREZ ALEXEI FEDOROVISH LICEA NAVARRO | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| http://dx.doi.org/10.3390/toxins8020039 | |
| Conus princeps, Calcium channel, Gamma-conotoxin, Mass spectrometry, Dorsal root ganglion neurons | |
| "A novel conotoxin, named as PiVIIA, was isolated from the venom of Conus princeps, a marine predatory cone snail collected in the Pacific Southern Coast of Mexico. Chymotryptic digest of the S-alkylated peptide in combination with liquid chromatography coupled to tandem mass spectrometry, were used to define the sequencing of this peptide. Eleven N-terminal amino acids were verified by automated Edman degradation. PiVIIA is a 25-mer peptide (CDAOTHYCTNYWγCCSGYCγHSHCW) with six cysteine residues forming three disulphide bonds, a hydroxyproline (O) and two gamma carboxyglutamic acid (γ) residues. Based on the arrangement of six Cys residues (C-C-CC-C-C), this conotoxin might belong to the O2-superfamily. Moreover, PiVIIA has a conserved motif (-γCCS-) that characterizes γ-conotoxins from molluscivorous Conus. Peptide PiVIIA has 45% sequence identity with γ-PnVIIA—the prototype of this family. Biological activity of PiVIIA was assessed by voltage-clamp recording in rat dorsal root ganglion neurons. Perfusion of PiVIIA in the µM range produces a significant increase in the Ca<sup>2+</sup> currents, without significantly modifying the Na<sup>+</sup>, K<sup>+</sup> or proton-gated acid sensing ionic currents. These results indicate that PiVIIA is a new conotoxin whose activity deserves further studies to define its potential use as a positive modulator of neuronal activity. View Full-Text" | |
| MDPI AG | |
| 2016 | |
| Artículo | |
| Toxins, Vol. 8, No. 2, Págs. 1-18 | |
| Inglés | |
| Bernáldez,J.,Jiménez,S.,González,L.J.,Noda Ferro,J.,Soto,E.,Salceda,E.,Chávez,D.,Aguilar,M.B.,Licea Navarro,A.2016.A New Member of Gamma-Conotoxin Family Isolated from Conus princeps Displays a Novel Molecular Target.Toxins,8(2),1-18.doi:10.3390/toxins8020039 | |
| BIOLOGÍA Y QUÍMICA | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos - Innovación Biomédica |
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