Please use this identifier to cite or link to this item: http://cicese.repositorioinstitucional.mx/jspui/handle/1007/1642
The carotenogenesis pathway via the isoprenoid-β-carotene interference approach in a new strain of Dunaliella salina isolated from Baja California Mexico
JOSE DE JESUS PANIAGUA MICHEL
Rémi Capa
JORGE OLMOS SOTO
LUIS ENRIQUE GUTIERREZ MILLAN
Acceso Abierto
Atribución-NoComercial-SinDerivadas
http://dx.doi.org/10.3390/md7010045
Bioactive compounds
"D. salina is one of the recognized natural sources to produce β-carotene, and an useful model for studying the role of inhibitors and enhancers of carotenogenesis. However there is little information in D. salina regarding whether the isoprenoid substrate can be influenced by stress factors (carotenogenic) or selective inhibitors which in turn may further contribute to elucidate the early steps of carotenogenesis and biosynthesis of β-carotene. In this study, Dunaliella salina (BC02) isolated from La Salina BC Mexico, was subjected to the method of isoprenoids-β-carotene interference in order to promote the interruption or accumulation of the programmed biosynthesis of carotenoids. When Carotenogenic and non-carotenogenic cells of D. salina BC02 were grown under photoautotrophic growth conditions in the presence of 200 µM fosmidomycin, carotenogenesis and the synthesis of β-carotene were interrupted after two days in cultured D. salina cells. This result is an indirect consequence of the inhibition of the synthesis of isoprenoids and activity of the recombinant DXR enzyme thereby preventing the conversion of 1-deoxy-D-xylulose 5-phosphate (DXP) to 2-C-methyl-D-erythritol (MEP) and consequently interrupts the early steps of carotenogenesis in D. salina. The effect at the level of proteins and RNA was not evident. Mevinolin treated D. salina cells exhibited carotenogenesis and β-carotene levels very similar to those of control cell cultures indicating that mevinolin not pursued any indirect action in the biosynthesis of isoprenoids."
MDPI AG
2009
Artículo
Marine Drugs, Vol. 7, Págs. 45-56
Inglés
Paniagua Michel,J.,Capa Robles,W.,Olmo Soto,J.,Gutiérrez Millán,L.E.2009.The Carotenogenesis Pathway via the Isoprenoid-β carotene Interference Approach in a New Strain of Dunaliella salina Isolated from Baja California Mexico.Marine Drugs,7,45-56.doi:10.3390/md7010045
CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA
Versión publicada
publishedVersion - Versión publicada
Appears in Collections:Artículos - Biotecnología Marina

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