Please use this identifier to cite or link to this item: http://cicese.repositorioinstitucional.mx/jspui/handle/1007/1652
A Conus regularis conotoxin with a novel eight-cysteine framework inhibits Cav2.2 channels and displays an anti-nociceptive activity
JOHANNA BERNALDEZ SARABIA
SERGIO AGUSTIN ROMAN GONZALEZ
OSCAR MARTÍNEZ GALVÁN
SAMANTA MIREYA JIMENEZ FLORES
OSCAR LEONARDO VIVAS RODRIGUEZ
Isabel Arenas
Gerardo Corzo
ROBERTO ALEJANDRO ARREGUIN ESPINOSA DE LOS MONTEROS
DAVID ERASMO GARCIA DIAZ
LOURIVAL DOMINGOS POSSANI POSTAY
ALEXEI FEDOROVISH LICEA NAVARRO
Acceso Abierto
Atribución-NoComercial-SinDerivadas
http://dx.doi.org/10.3390/md11041188
Conus regularis, Nociceptive, Calcium channel, Eight-cysteine toxin and conotoxins
"A novel peptide, RsXXIVA, was isolated from the venom duct of Conus regularis, a worm-hunting species collected in the Sea of Cortez, México. Its primary structure was determined by mass spectrometry and confirmed by automated Edman degradation. This conotoxin contains 40 amino acids and exhibits a novel arrangement of eight cysteine residues (C-C-C-C-CC-CC). Surprisingly, two loops of the novel peptide are highly identical to the amino acids sequence of ω-MVIIA. The total length and disulfide pairing of both peptides are quite different, although the two most important residues for the described function of ω-MVIIA (Lys2 and Tyr13) are also present in the peptide reported here. Electrophysiological analysis using superior cervical ganglion (SCG) neurons indicates that RsXXIVA inhibits CaV2.2 channel current in a dose-dependent manner with an EC50 of 2.8 μM, whose effect is partially reversed after washing. Furthermore, RsXXIVA was tested in hot-plate assays to measure the potential anti-nociceptive effect to an acute thermal stimulus, showing an analgesic effect in acute thermal pain at 30 and 45 min post-injection. Also, the toxin shows an anti-nociceptive effect in a formalin chronic pain test. However, the low affinity for CaV2.2 suggests that the primary target of the peptide could be different from that of ω-MVIIA."
MDPI AG
2013
Artículo
Marine Drugs, Vol. 11, Págs. 1188-1202
Inglés
Bernáldez,J.,Román González,S.A.,Martínez,O.,Jiménez,S.,Vivas,O.,Arenas,I.,Corzo,G.,Arreguín,R.,García,D.E.,Possani,L.D.,Licea,A.2013.A Conus regularis Conotoxin with a Novel Eight-Cysteine Framework Inhibits CaV2.2 Channels and Displays an Anti-Nociceptive Activity.Marine Drugs,11,1188-1202.doi:10.3390/md11041188
CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA
Versión publicada
publishedVersion - Versión publicada
Appears in Collections:Artículos - Biotecnología Marina

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