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Fluorescence in situ hybridization of rDNA, telomeric (TTAGGG)n and (GATA)n repeats in the red abalone Haliotis rufescens (Archaeogastropoda: Haliotidae)
Cristian Gallardo
JOSUE ALVAREZ BORREGO
MIGUEL ANGEL DEL RIO PORTILLA
Ismael Cross
Alvaro Merlo
LAUREANA REBORDINOS
Acceso Abierto
Atribución-NoComercial-SinDerivadas
http://dx.doi.org/10.1111/j.1601-5223.2005.01909.x
Chromosomal Location, Cytogenetic Characterization, Mytilus-Galloprovincialis, Population-Genetics, Crassostrea-Gigas, Pacific Abalone, Donax-Trunculus, Ribosomal Dna, Black Abalone, California
"The physical location of 18S-5.8S-28S rDNA, telomeric sequences with (TTAGGG)n DNA probe and (GATA)<sub>n</sub> microsatellites were performed by fluorescence in situ hybridization in chromosomes of red abalone Haliotis rufescens. The karyotype of red abalone showed a diploid number of 36 (8M+9SM+1ST). FISH performed with rDNA probe, showed the location of major ribosomal clusters in the terminal region of the large arms of two submetacentric pairs (chromosome 4 and 5). Localization of heteromorphisms of FISH-rDNA was found between chromosome homologues and sister chromatids in all metaphases analyzed. This indicates that rDNA clusters are variable within the red abalone genome. The variability in the NOR-bearing reported using silver staining in other gastropods and our result are discussed. In addition, the presence of microsatellite (TTAGGG)<sub>n</sub> and (GATA)n was demonstrated after FISH treatment by DNA probes. The telomeric sequence occurred at the ends of all mitotic chromosomes, while the (GATA)<sub>n</sub> repetitive was found on chromosomal interstitial zones as well as at the telomeres in abalone chromosomes."
BioMed Central
2005
Artículo
Hereditas, Vol. 142, Págs. 73-79
Inglés
Gallardo Escárate,C.,Álvarez Borrego,J.,Del Río Portilla,M.A.,Cross,I.,Merlo,A.,Rebordinos,L.2005.Fluorescence in situ hybridization of rDNA, telomeric (TTAGGG)<sub>n</sub> and (GATA)<sub>n</sub> repeats in the red abalone Haliotis rufescens (Archaeogastropoda: Haliotidae).HereditasHereditas,142,73-79.doi:10.1111/j.1601-5223.2005.01909.x
ÓPTICA
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