Please use this identifier to cite or link to this item: http://cicese.repositorioinstitucional.mx/jspui/handle/1007/1748
Litopenaeus vannamei immunestimulated with Macrocystis pyrifera extract: improving the immune response against Vibro campbellii
LILIANA NOEMI SANCHEZ CAMPOS
FERNANDO DIAZ HERRERA
ANA DENISE RE ARAUJO
Ricardo González
MARCIAL LEONARDO LIZARRAGA PARTIDA
MANUEL DE JESUS ACOSTA RUIZ
ALEXEI FEDOROVISH LICEA NAVARRO
Acceso Abierto
Atribución-NoComercial-SinDerivadas
http://dx.doi.org/10.12980/JCLM.2.2014JCLM-2014-0021
Immune stimulation, Hot-water extract, Clearance rate, Antimicrobial activity, Seaweed, Vibrio campbellii, Litopenaeus vannamei, Macrocystis pyrifera, PCR
"Objective: To examine the immune responses of Litopenaeus vannamei after different treatments with a hot water extract of Macrocystis pyrifera (M. pyrifera) and a subsequent challenge with Vibrio campbellii (V. campbellii). Methods: A total of 184 adult white shrimp that were infected with V. campbellii (1X10<sup>6</sup> CFU/ shrimp) were immunostimulate by the hot-water extract from M. pyrifera via either injection (10 µg) or immersion (350 mg/L), the experimental controls were injected with either saline solution or V. campbellii (1X10<sup>6</sup> CFU/shrimp). The bacterial DNA depuration rate, antimicrobial activity and total hemocyte count were evaluated in hemolymph samples at 2, 6, 12, 24, 48 and 72 h postinfection. Results: Injected shrimp (10 µg M. pyrifera extract) demonstrated the best clearance of bacterial infection, with 82% survival at 72 h post-infection (cellular response). Hemolymph from the immersed organisms had the best antimicrobial activity against Escherichia coli growth; specifically, the most efficient antimicrobial activity was observed at 24 h post-infection. Both types of immunostimulated shrimp had similar total hemocyte counts at 24 h post-infection (1.63- 1.59 million/mL); however, after 72 h, injected shrimp had higher total hemocyte counts than immersed animals (2.59 v.s. 0.56 million/mL). Conclusions: The injection of the M. pyrifera hot-water extract facilitated a more efficient response to V. campbellii infection due to the stimulation of the hemocytes of the shrimp. In other words, the cellular immune response was more efficient to eliminate bacterial infection than the humoral response in shrimp."
Journal of Coastal Life Medicine Editorial Office
2014
Artículo
Journal of Coastal Life Medicine, Vol. 2, No. 8, Págs. 617-624
Inglés
Sánchez Campos,L.N.,Díaz Herrera,F.,Re AraujoA.D., González Sánchez,R.A.,Lizárraga Partida,M.L.,Acosta Ruíz,M.J.,Licea Navarro,A.F.2014.Litopenaeus vannamei immunestimulated with Macrocystis pyrifera extract: improving the immune response against Vibrio campbellii.Journal of Coastal Life Medicine,2(8),617-624.doi:10.12980/JCLM.2.2014JCLM-2014-0021
CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA
Versión publicada
publishedVersion - Versión publicada
Appears in Collections:Artículos - Biotecnología Marina

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